what is the melting point of AAGCCTAGAGAGATCA

oligonucleotide melting temperature | oligonucleotide sequence | AAGCCTAGAGAGATCA (genome sequence) monovalent salt ion concentration | 0.05 mol/L (moles per liter) magnesium ion concentration | 2.5 mmol/L (millimoles per liter) primer concentration | 0.4 µmol/L (micromoles per liter)

oligonucleotide melting temperature | 52.1 °C (degrees Celsius) (based on Wetmur-Sninsky nearest-neighbor base interaction model)

T_m = T_t + T_s - 269.3 | T_t = (T^° ΔH^°)/(ΔH^° - ΔG^° + R T^° log(C)) T_s = 16.6 log(10, [salt]/(1 + 0.7 [salt])) | [salt] = [K^+] + 4 sqrt([Mg^(2+)]) | T_m | melting temperature T_t | thermodynamic term T_s | salt-dependent term T^° | STP temperature (25°C) ΔH^° | base-stacking enthalpy ΔG^° | base-stacking free energy R | molar gas constant C | primer concentration [salt] | effective salt concentration [K^+] | monovalent salt concentration [Mg^(2+)] | magnesium ion concentration

Salt concentration dependence

16 base pairs

(5'-3' frame 1) | AAG | CCU | AGA | GAG | AUC ↓ | ↓ | ↓ | ↓ | ↓ K | P | R | E | I (5'-3' frame 2) | AGC | CUA | GAG | AGA | UCA ↓ | ↓ | ↓ | ↓ | ↓ S | L | E | R | S (5'-3' frame 3) | GCC | UAG | AGA | GAU ↓ | ↓ | ↓ | ↓ A | - | R | D (3'-5' frame 1) | UGA | UCU | CUC | UAG | GCU ↓ | ↓ | ↓ | ↓ | ↓ - | S | L | - | A (3'-5' frame 2) | GAU | CUC | UCU | AGG | CUU ↓ | ↓ | ↓ | ↓ | ↓ D | L | S | R | L (3'-5' frame 3) | AUC | UCU | CUA | GGC ↓ | ↓ | ↓ | ↓ I | S | L | G